LIAO Rijing
Associate Professor, Manager for Mass Spectroscopy
Research direction：Novel methods for quantitative characterization of proteomes in complex biological samples
E-mail：rjliao@@shsmu.edu.cn
Research Interests
Based on the liquid chromatography-mass spectrometry, we focus on the development of novel methods for quantitative analysis of the proteomes in biological samples, such as cell lines and tissues.
Stable isotopic labeling of proteins/peptides through chemical reactions has become widely used in quantitative proteomics studies, as this strategy minimizes the technical variability in sample preparation and MS analysis. However, chemical labeling often causes undesired side reactions, compromising the analytical sensitivity and dynamic range. We attempt to investigate the chemical mechanism of the side reactions and develop novel labeling methods allowing specific and efficient labeling of proteins/peptides.
Histones play a crucial role in the epigenetic regulation via its various post-translational modifications (PTMs). However, deciphering histone PTMs, especially their combinatorial patterns, by LC-MS remains an area of active development. Based on the histone derivatization method we established previously,，we attempt to establish novel analytical method for accurate and robust characterization of histone PTMs on both bottom-up and middle-up approaches.
Education and Work Experience
2000-9 to 2004-6, East China Normal University, Chemistry (BSc)
2006-9 to 2011-7, Shanghai Institute of Organic Chemistry ACS, organic chemistry (PhD)
2011-7 to 2015-5, Novartis (China) Institute for Biomedical Research, Analytical Chemistry, research fellow
2015-6 to 2018-2, ACS Shanghai Clinical Research Center, Associate Professor
2018-3 to now, Shanghai Institute of Precision Medicine, Associate Professor
Selected Publications
Bo Pang, Rijing Liao, Zhijun Tang, Shengjie Guo, Zhuhua Wu, Wen Liu*. Caerulomycin and collismycin antibiotics share a trans-acting flavoprotein-dependent assembly line for 2,2’-bipyridine formation. Nature Communications, 2021, 12:3124.
Rijing Liao*, Yuan Gao, Ming Chen, Lulu Li, Xuye Hu. A Ubiquitous but Overlooked Side Reaction in Dimethyl Labeling of Peptides, Analytical Chemistry, 2018, 90: 13533-13540.
Liao Rijing*, Zheng Dan, Nie Aiying, Zhou Shaolian, Deng Haibing, Gao Yuan, Yang Pengyuan, Yu Yanyan, Tan Lin, Qi Wei, Wu Jiaxi, Li En, Yi Wei*. Sensitive and Precise Characterization of Combinatorial Histone Modifications by Selective Derivatization Coupled with RPLC-EThcD-MS/MS., J Proteome Res, 2017, 16(2): 780-787.
Liao Rijing, Wu Haiping, Deng Haibing, Yu Yanyan, Hu Min, Zhai Huili, Yang Pengyuan, Zhou Shaolian*, Yi Wei*. Specific and efficient N-propionylation of histones with propionic acid N-hydroxysuccinimide ester for histone marks characterization by LC-MS., Analytical Chemistry, 2013, 85: 2253-2259.
