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師資隊(duì)伍
金穎
  作者:  2018-12-31

基本信息 >>>

2321D

 金穎
  組織胚胎學(xué)與遺傳發(fā)育學(xué)系
  電話:
021-54923342
  郵箱:[email protected]

研究方向>>>

多年來集中研究多能干細(xì)胞,尤其是人胚胎干細(xì)胞,在體外增殖和分化的調(diào)控機(jī)制,致力于揭示維持胚胎干細(xì)胞無限自我更新的信號(hào)通路、轉(zhuǎn)錄因子和表觀遺傳調(diào)控因子等,旨在優(yōu)化人胚胎干細(xì)胞的體外培養(yǎng)體系。同時(shí),還關(guān)注人胚胎干細(xì)胞如何定向分化為神經(jīng)細(xì)胞,尤其是如何形成神經(jīng)系統(tǒng)不同區(qū)域的神經(jīng)祖細(xì)胞,為獲得臨床疾病治療需要的功能細(xì)胞奠定基礎(chǔ)。

個(gè)人簡歷 >>>

金穎,研究員,博士生導(dǎo)師。中國醫(yī)學(xué)科學(xué)院/北京協(xié)和醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)研究所病理生理學(xué)/藥理學(xué)博士,美國北德克薩斯州大學(xué)健康科學(xué)中心Research Associate (1988-1994),美國德克薩斯大學(xué)西南醫(yī)學(xué)中心Research Fellow (1994-1998)。上海交通大學(xué)特聘教授,上海交通大學(xué)基礎(chǔ)醫(yī)學(xué)院特聘教授,中國科學(xué)院上海營養(yǎng)與健康研究所課題組長。上海曙光學(xué)者、中國科學(xué)院百人計(jì)劃、第二屆百名華僑華人專業(yè)人士杰出創(chuàng)業(yè)獎(jiǎng)、上海市優(yōu)秀學(xué)科帶頭人、上海市三八紅旗手、上海市領(lǐng)軍人才、上海交通大學(xué)優(yōu)秀共產(chǎn)黨員、中國科學(xué)院朱李月華優(yōu)秀教師和杰出研究員等榮譽(yù)獲得者。主要研究方向?yàn)槎嗄芨杉?xì)胞命運(yùn)決定的分子調(diào)控。先后任J Biol Chem雜志編委,Stem Cell Research and Therapy雜志編委,《上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》編委,《中華細(xì)胞與干細(xì)胞雜志》副主編,國際干細(xì)胞研究學(xué)會(huì)(ISSCR)會(huì)員、干細(xì)胞研究和臨床轉(zhuǎn)化指南更新任務(wù)組成員(2014-2016年),上海市細(xì)胞生物學(xué)學(xué)會(huì)理事,中國細(xì)胞生物學(xué)學(xué)會(huì)細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)分會(huì)委員,中國細(xì)胞生物學(xué)學(xué)會(huì)干細(xì)胞生物學(xué)分會(huì)副會(huì)長等。在Cell Stem Cell Cell ResDev CellJ Clin InvestPNAS等學(xué)術(shù)期刊上發(fā)表通訊作者論文70余篇,先后獲得教育部自然科學(xué)獎(jiǎng)二等獎(jiǎng)、上海市科學(xué)技術(shù)獎(jiǎng)二等獎(jiǎng)和三等獎(jiǎng),以及中國干細(xì)胞研究創(chuàng)新獎(jiǎng)。

科研項(xiàng)目 >>>

1. 31730055轉(zhuǎn)錄后選擇性剪接相關(guān)基因C20ORF14在人胚胎干細(xì)胞自我更新維持中的功能和機(jī)制研究,國家自然科學(xué)基金重點(diǎn)項(xiàng)目,2018.01-2022.12342.4萬元,在研,項(xiàng)目負(fù)責(zé)人金穎;

2. WF410170022上海交通大學(xué)雙一流建設(shè)配套項(xiàng)目,2018.07-2021.06280萬元,在研,項(xiàng)目負(fù)責(zé)人金穎;

3. 2016YFA0100100多能干細(xì)胞自我更新與維持的調(diào)控機(jī)制研究,科技部國家重點(diǎn)研發(fā)計(jì)劃干細(xì)胞及轉(zhuǎn)化研究重點(diǎn)專項(xiàng),2016.07-2021.062789萬元,在研,項(xiàng)目負(fù)責(zé)人金穎;

4. 19ZR1428300VEGF信號(hào)通路在維持人胚胎干細(xì)胞自我更新和全能性中的功能和分子機(jī)制,上海市科委自然科學(xué)基金,2019.07-2022.0620萬元,在研,項(xiàng)目負(fù)責(zé)人李慧;

5. 16PJD031Oct4泛素連接酶在早期胚胎發(fā)育過程的功能與機(jī)制研究,上海市浦江人才計(jì)劃,2016.9-2018.820萬元,已結(jié)題,項(xiàng)目負(fù)責(zé)人廖兵;

6. 31471393轉(zhuǎn)錄因子SOX2在人胚胎干細(xì)胞和神經(jīng)祖細(xì)胞中的重要作用及機(jī)制研究,國家自然科學(xué)基金面上項(xiàng)目,2014.01-2018.1290萬元,已結(jié)題,項(xiàng)目負(fù)責(zé)人金穎;

7. 31471231Oct4泛素連接酶在發(fā)育多能性的建立和維持中的作用及機(jī)制研究,國家自然科學(xué)基金面上項(xiàng)目,2014.01-2018.1280萬元,已結(jié)題,項(xiàng)目負(fù)責(zé)人廖兵;

8. 31271456轉(zhuǎn)錄共激活因子Citedl促進(jìn)胚胎干細(xì)胞向滋養(yǎng)層分化,國家自然科學(xué)基金面上項(xiàng)目,2012.01-2016.1280萬元,已結(jié)題,項(xiàng)目負(fù)責(zé)人李慧。

論文與專著 >>>

作者 論文標(biāo)題 期刊名 出版年 卷期頁碼
Fang Z, Liu X, Wen J, Tang F, Zhou Y, Jing N, Jin Y* SOX21 ensures rostral forebrain identity by suppression of WNT8B during neural regionalization of human embryonic stem cells. Stem Cell Reports. 2019 13: 1038-1052
He K, Hu J. Yu H, Wang L, Tang F, Gu J, Ge L, Wang H, Li S, Hu P and Jin Ying*  Serine/threonin kinase 40 (Stk40) functions as a novel regulator of skeletal muscle differentiation.  J Biol Chem   2017  292(1):351-360 
Yang J, Zhao H, Ma Y, Shi G, Song J, Tang U, Li S, Li T, Liu N, Tang F, Gu J, Zhang L, Zhang Z, Zhang X,  Early pathogenic event of Alzheimer’s disease documented in iPSCs from patients with PSEN1 mutations.   Oncotarget  2017  8(5):7900-7913. 
Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, Ge L, Tang F, Huang X, Zhou C, Wang P, Zheng D and Jin Y*  PHB associates with the HIRA complex to control an epigenetic-metabolic circuit in human ESCs.  Cell Stem Cell  2017  20(2): 274-289 
Zhou C, Yang X, Sun Y, Yu H, Zhang Y* and Jin Y*  Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.  Cell Res.  2016  26:171-189 
Li C, Ma Y, Zhang K, Gu J, Tang F, Chen S, Cao L*, Li S* and Jin Y*.   Aberrant tranional networks in step-wise neurogenesis of paroxysmal kinesigenic dyskinesia-induced pluripotent stem cells.  Oncotarget  2016  7(33):53611-53627. 
Fang L, Zhang J, Zhang H, Yang X, Jin X, Zhang L, Skalnik D,, Zhang Y, Huang X, Li J, Wong J*.   H3K4 methyltransferase Set1a is a key Oct4 coactivator essential for generation of Oct4 positive inner cell mass.  Stem Cells   2016  34(3):565-580. 
Zheng S, Sun M, Zhang K*, Gu J, Guo Z, Tian S, Zhai G, He X,, Zhang Y*.   Profiling post-translational modifications of histones in neural differentiation of embryonic stem cells using liquid chromatography-mass spectrometry.   J Chromatogr B   2016  1017-1018 :36-44. 
Ju X, Hou Q, Sheng A, Wu K, Zhou Y,, Wen T, Yang Z, Wang X, Luo Z*.   The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice.  Elife   2016  5: e18197-18222. 
Liu J, Luo X, Xu Y, Gu J, Tang F,  Single-stranded DNA binding protein Ssbp3 induces differentiation of mouse embryonic stem cells into trophoblast-like cells.   Stem Cell Res Ther   2016  7(1): 79-93. 
Qu Z,GuanY,Cui L, Song J, Gu J, Zhao H, Xu L, Lu L,  Transplantation of rat embryonic stem cell-derived retinal progenitor cells preserves the retinal structure and function in rat retinal degeneration.  Stem Cell Research & Therapy  2015  2015 6:219  
Sun M, Liao B, Tao Y, Chen H, Xiao F, Gu JJ, Gao SR and Jin Y*  Calcineurin-NFAT Signaling Controls Somatic Cell Reprogramming in a Stage-Dependent Manner.  Journal of Cellular Physiology  2015  9999: 1–12 
Yu H, He K, Wang L, Hu J, Gu J, Zhou C, Lu R,  Stk40 represses adipogenesis through translational control of CCAAT/enhancer-binding proteins.  J Cell Sci.  2015  128(15):2881-90. 
Wei Z, Sun M, Liu X, Zhang J,  Rufy3, a protein specifically expressed in neurons, interacts with actin-bundling protein Fascin to control the growth of axons.  J Neurosci Res  2014  92(5):607-18 
Zhu L, Zhang S,  Foxd3 suppresses NFAT-mediated differentiation to maintain self-renewal of embryonic stem cells.  EMBO Rep.  2014  15(12):1286-96 
Luo X, Wang B, Tang F, Zhang J, Zhao Y, Li H,  Wwp2 targets SRG3, a scaffold protein of the SWI/SNF-like BAF complex, for ubiquitination and degradation.  Biochem Biophys Res Commun.  2014  17;443(3):1048-53 
Liao B, Zhong X, Xu H, Xiao F, Fang Z, Gu J, Chen Y, Zhao Y,  Itch, an E3 ligase of Oct4, is required for embryonic stem cell self-renewal and pluripotency induction.  J Cell Physiol  2013  228(7):1443-51 
Zhou Y, Jiang H, Gu J,Tang Y, Shen N and Jin Y*.  MicroRNA-195 targets ADP-ribosylation factor like protein 2 inducing apoptosis in human embryonic stem cell-derived neural progenitor cells.  Cell Death & Disease  2013  4:e695 
Cui L, Guan Y, Qu Z, Zhang J, Liao B, Ma B, Qian J, Li D, Li W, Xu GT,  WNT signaling determines tumorigenicity and function of ESC-derived retinal progenitors.  J Clin Invest.  2013  123(4):1647-61 
Yu H, He K, Li L, Sun L, Tang F, Li R, Ning W,  Deletion of STK40 protein in mice causes respiratory failure and death at birth.  J Biol Chem  2013  288(8):5342-52 
Yang H, Liu Z, Ma Y, Zhong C, Yin Q, Zhou C, Shi D, Cai Y, Zhao H, Wang H, Tang F, Wang Y, Zhang C, Lai D,, Sun Q*, Li J*  Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes  Cell Res   2013  23:1187-1200 
Guan Y, Cui L, Qu Z, Lu L, Wang F, Wu Y, Zhang J, Gao F, Tian H, Xu L, Xu G, Li W*,  Subretinal transplantation of rat MSCs and erythropoietin gene modified rat MSCs for protecting and rescuing degenerative retina in rats  Cur Mol Med   2013  13:1419-1431 
Ma Y, Gu J, Li C, Wei X, Tang F, Shi G, Jiang J, Kuang J, Li J, Wang Z, Xie X ,  Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells  Stem Cell Research & Therapy  2012  3(4):29 
Ma Y, Li C, Gu J, Tang F, Li C, Li P, Ping P, Yang S, Li Z,  Aberrant gene expression profilesin pluripotent stem cells induced from fibroblasts of a klinefelter syndrome patient  J Biol Chem.  2012  287(46): 38970–38979 
Zhang H, Ma Y, Gu J, Liao B, Li J, Wong J,  Reprogramming of somatic cells via TAT-mediated protein transduction of recombinant factors  Biomaterials  2012  33(20): 5047- 5055 
Zhou Y,  Differentiation of Human Embryonic Stem Cells into Neural Lineage Cells. In M.A. Hayat (ed.)  Stem Cells and Cancer Stem Cells.  2012  7:229-239 
Shi G,Gao F,  The regulatory role of histone deacetylase inhibitors in Fgf4 expression is dependent on the differentiation state of pluripotent stem cells  J Cell Physiol.  2011  226:3190-3196. 
Lu R, Yang A,  Dual functions of T-box3 in the control of self-renewal and extraembryonic endoderm differentiation in mouse embryonic stem cells.  J Biol Chem.  2011  286 (10):8425-8436. 
Yang A, Shi G, Zhou C, Lu R, Li H ,Sun L,  Nucleolin Maintains Embryonic Stem Cell Self-renewal by Suppression of p53 Protein-dependent Pathway  J Biol Chem.  2011  286(50):43370-43382 
Li X, Zhu L, Yang A, Lin J, Tang F, Jin S, Wei Z, Li J,  Calcineurin-NFAT signaling critical regulates early lineage specification in mouse embryonic stem cells and embryos  Cell Stem Cell.  2011  8:46-58. 
Fang H, Yang Y, Li C, Fu S, Yang Z, Jin G, Wang K, Zhang J,  Tranome analysis of early organogenesis in human embryos.  Developmental Cell  2010  19:174-184. 
Li L, Sun L, Gao F, Jiang J, Yang Y, Li C, Gu J, Wei Z, Yang A, Lu R, Ma Y, Tang F, Kwon S, Zhao Y, Li S,  Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation.   PNAS  2010  107 (4):1402-1407 
Shi G,  Role of Oct4 in maintaining and regaining stem cell pluripotency.   Stem Cell Research & Therapy  2010  1(5): 39 
Li C, Yang Y, Lu X, Sun Y, Gu J, Feng Y,  Efficient derivation of Chinese human embryonic stem cell lines from frozen embryos.  In vitro Cell & Dev. Biol. –Animal  2010  46:186-191. 
Liao B,  Wwp2 mediates Oct4 ubiquitination and its auto-ubiquitination in a dosage-dependent manner.  Cell Res.  2010  20(3):332-344. 
Li C, Yang Y, Gu J, Ma Y,  Derivation and tranional profiling analysis of pluripotent stem cell lines from rat blastocysts  Cell Res  2009  19:173-186 
Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, Yang Y, Jin S, Wei Z, Jiang H, Li J,  Germline-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor.  PLoS One  2009  4:e6724 
Chunliang Li ,,   Pluripotency can be repidly and efficiently induced in human amniotic fluid-dsrived cells   Human Molecular Genetics  2009  15(22): 4340-9 
Li H, Wang B, Yang A, Lu R, Wang W,  LYAR is an important nucleolar protein for control of self-renewal and differentiation in embryonic stem cells  Stem Cells  2009  5;27(6):1244-1254 
Xu H, Wang W, Li C, Yu H, Yang A, Wang B,  WWP2 promotes degradation of tranion factor OCT4 in human embryonic stem cells  Cell Res   2009  19:561-573 
Zhong X,  Critical roles of coactivator p300 in mouse embryonic stem cell differentiation and Nanog expression.   Journal of Biological Chemistry  2009  284 (14 ) :9186-9175 
Lingjie Li,Lei Sun,  Stk40 links the pluripotency factor Oct4 to the Erk/MAPK Pathway and controls extraembryonic endoderm differentiation   PNAS  2009  107 (4):1402-1407 
Furong Gao,  PARP1poly(ADP-ribosyl)ates Sox2 to control Sox2 protein levels and FGF4 expression during embryonic stem cell differentiation   Journal of Biological Chemistry  2009  14;284(33): 22263-73. 

專利與技術(shù)許可 >>>

 

1. Oct4泛素化修飾突變體在提高誘導(dǎo)體細(xì)胞重編程效率中的應(yīng)用,申請(qǐng)?zhí)?/span>201810783982.4,金穎,廖兵,李爽,肖峰;

2. 一種PRRT2蛋白相關(guān)的抗原表位肽及其應(yīng)用,申請(qǐng)?zhí)?/span>2014103873851,金穎、李春;

3. 一種調(diào)控誘導(dǎo)產(chǎn)生的視網(wǎng)膜前體細(xì)胞的方法,ZL 2012 1 0279963.0,金穎、徐國彤、崔璐、管圓;

4. 抗小鼠Stk40多克隆抗體及其制備,ZL 2011 1 0214235.7,金穎、楊阿聰;

5. 調(diào)控胚胎干細(xì)胞及早期胚胎的胚層分化的方法和試劑,ZL 2010 1 0268307.1,金穎、李翔、朱莉莉。 

 

榮譽(yù)與獎(jiǎng)勵(lì) >>>

1. 2001年上海曙光學(xué)者;

2. 2004年度獲得中國科學(xué)院“百人計(jì)劃”支持;

3. 2006年當(dāng)選為上海市優(yōu)秀學(xué)科帶頭人;

4. 2009年度第二屆百名華僑華人專業(yè)人士-杰出創(chuàng)業(yè)獎(jiǎng);

5. 2009-2010年度獲上海市“三八”紅旗手稱號(hào);

6. 2010年度中國科學(xué)院“朱李月華優(yōu)秀教師”獎(jiǎng);

7. 2013年度、2016年度中國科學(xué)院上海生命科學(xué)研究院/上海交通大學(xué)醫(yī)學(xué)院健康科學(xué)研究所“杰出研究員”獎(jiǎng);

8. 2011年度、2012年度、2017年度中國科學(xué)院“優(yōu)秀研究生指導(dǎo)教師”獎(jiǎng);

9. 2011年度、2016年度中國科學(xué)院上海生命科學(xué)研究院/上海交通大學(xué)醫(yī)學(xué)院健康科學(xué)研究所優(yōu)秀共產(chǎn)黨員;

10. “胚胎干細(xì)胞及早期胚胎發(fā)育的分子調(diào)控機(jī)制研究”獲得2012年教育部自然科學(xué)獎(jiǎng)二等獎(jiǎng)(第一完成人);

11. “胚胎干細(xì)胞中Oct4轉(zhuǎn)錄因子的分子調(diào)控機(jī)制研究”獲得2013年獲得上海市科學(xué)技術(shù)獎(jiǎng)三等獎(jiǎng)(第一完成人);

12. “以多能干細(xì)胞為模型對(duì)細(xì)胞命運(yùn)決定分子機(jī)制的研究”獲得2014年上海市自然科學(xué)獎(jiǎng)二等獎(jiǎng)(第一完成人);

13. 2017年、2018年實(shí)驗(yàn)室獲得中國細(xì)胞生物學(xué)學(xué)會(huì)全國聯(lián)動(dòng)實(shí)驗(yàn)室開放日科普活動(dòng)優(yōu)秀獎(jiǎng);

14. 2017年中國細(xì)胞生物學(xué)學(xué)會(huì)干細(xì)胞生物學(xué)分會(huì)干細(xì)胞研究創(chuàng)新獎(jiǎng);

15. 2018年度上海交通大學(xué)基礎(chǔ)醫(yī)學(xué)院優(yōu)秀員工獎(jiǎng)。