基本信息 >>>
研究方向>>>
多年來集中研究多能干細胞,，尤其是人胚胎干細胞,，在體外增殖和分化的調(diào)控機制,，致力于揭示維持胚胎干細胞無限自我更新的信號通路,、轉(zhuǎn)錄因子和表觀遺傳調(diào)控因子等,，旨在優(yōu)化人胚胎干細胞的體外培養(yǎng)體系,。同時,，還關注人胚胎干細胞如何定向分化為神經(jīng)細胞，尤其是如何形成神經(jīng)系統(tǒng)不同區(qū)域的神經(jīng)祖細胞,，為獲得臨床疾病治療需要的功能細胞奠定基礎,。 |
個人簡歷 >>>
金穎，研究員,，博士生導師,。中國醫(yī)學科學院/北京協(xié)和醫(yī)科大學基礎醫(yī)學研究所病理生理學/藥理學博士，美國北德克薩斯州大學健康科學中心Research Associate (1988-1994),，美國德克薩斯大學西南醫(yī)學中心Research Fellow (1994-1998),。上海交通大學特聘教授，上海交通大學基礎醫(yī)學院特聘教授,，中國科學院上海營養(yǎng)與健康研究所課題組長,。上海曙光學者、中國科學院“百人計劃”,、第二屆百名華僑華人專業(yè)人士杰出創(chuàng)業(yè)獎,、上海市優(yōu)秀學科帶頭人,、上海市“三八”紅旗手、上海市領軍人才,、上海交通大學優(yōu)秀共產(chǎn)黨員,、中國科學院 “朱李月華優(yōu)秀教師”和杰出研究員等榮譽獲得者。主要研究方向為多能干細胞命運決定的分子調(diào)控,。先后任J Biol Chem雜志編委,，Stem Cell Research and Therapy雜志編委，《上海交通大學學報（醫(yī)學版）》編委,，《中華細胞與干細胞雜志》副主編,，國際干細胞研究學會（ISSCR）會員、干細胞研究和臨床轉(zhuǎn)化指南更新任務組成員（2014年-2016年）,，上海市細胞生物學學會理事,，中國細胞生物學學會細胞信號轉(zhuǎn)導分會委員，中國細胞生物學學會干細胞生物學分會副會長等,。在Cell Stem Cell,、 Cell Res、Dev Cell,、J Clin Invest和PNAS等學術期刊上發(fā)表通訊作者論文70余篇,，先后獲得教育部自然科學獎二等獎、上海市科學技術獎二等獎和三等獎,，以及中國干細胞研究創(chuàng)新獎,。 |
科研項目 >>>
1. 31730055轉(zhuǎn)錄后選擇性剪接相關基因C20ORF14在人胚胎干細胞自我更新維持中的功能和機制研究，國家自然科學基金重點項目,，2018.01-2022.12,，342.4萬元，在研,，項目負責人金穎,； 2. WF410170022上海交通大學“雙一流”建設配套項目，2018.07-2021.06,，280萬元,，在研，項目負責人金穎,； 3. 2016YFA0100100多能干細胞自我更新與維持的調(diào)控機制研究,，科技部國家重點研發(fā)計劃“干細胞及轉(zhuǎn)化研究”重點專項，2016.07-2021.06,，2789萬元,，在研，項目負責人金穎,； 4. 19ZR1428300VEGF信號通路在維持人胚胎干細胞自我更新和全能性中的功能和分子機制,，上海市科委自然科學基金,，2019.07-2022.06，20萬元,，在研,，項目負責人李慧； 5. 16PJD031Oct4泛素連接酶在早期胚胎發(fā)育過程的功能與機制研究,，上海市浦江人才計劃，2016.9-2018.8,，20萬元,，已結題，項目負責人廖兵,； 6. 31471393轉(zhuǎn)錄因子SOX2在人胚胎干細胞和神經(jīng)祖細胞中的重要作用及機制研究,，國家自然科學基金面上項目，2014.01-2018.12,，90萬元,，已結題，項目負責人金穎,； 7. 31471231Oct4泛素連接酶在發(fā)育多能性的建立和維持中的作用及機制研究,，國家自然科學基金面上項目，2014.01-2018.12,，80萬元,，已結題，項目負責人廖兵,； 8. 31271456轉(zhuǎn)錄共激活因子Citedl促進胚胎干細胞向滋養(yǎng)層分化,，國家自然科學基金面上項目，2012.01-2016.12,，80萬元,，已結題，項目負責人李慧,。
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論文與專著 >>>
| Fang Z, Liu X, Wen J, Tang F, Zhou Y, Jing N, Jin Y* |
SOX21 ensures rostral forebrain identity by suppression of WNT8B during neural regionalization of human embryonic stem cells. |
Stem Cell Reports. |
2019 |
13: 1038-1052 |
| He K, Hu J. Yu H, Wang L, Tang F, Gu J, Ge L, Wang H, Li S, Hu P and Jin Ying* |
Serine/threonin kinase 40 (Stk40) functions as a novel regulator of skeletal muscle differentiation. |
J Biol Chem |
2017 |
292(1):351-360 |
| Yang J, Zhao H, Ma Y, Shi G, Song J, Tang U, Li S, Li T, Liu N, Tang F, Gu J, Zhang L, Zhang Z, Zhang X, |
Early pathogenic event of Alzheimer’s disease documented in iPSCs from patients with PSEN1 mutations. |
Oncotarget |
2017 |
8(5):7900-7913. |
| Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, Ge L, Tang F, Huang X, Zhou C, Wang P, Zheng D and Jin Y* |
PHB associates with the HIRA complex to control an epigenetic-metabolic circuit in human ESCs. |
Cell Stem Cell |
2017 |
20(2): 274-289 |
| Zhou C, Yang X, Sun Y, Yu H, Zhang Y* and Jin Y* |
Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs. |
Cell Res. |
2016 |
26:171-189 |
| Li C, Ma Y, Zhang K, Gu J, Tang F, Chen S, Cao L*, Li S* and Jin Y*. |
Aberrant tranional networks in step-wise neurogenesis of paroxysmal kinesigenic dyskinesia-induced pluripotent stem cells. |
Oncotarget |
2016 |
7(33):53611-53627. |
| Fang L, Zhang J, Zhang H, Yang X, Jin X, Zhang L, Skalnik D,, Zhang Y, Huang X, Li J, Wong J*. |
H3K4 methyltransferase Set1a is a key Oct4 coactivator essential for generation of Oct4 positive inner cell mass. |
Stem Cells |
2016 |
34(3):565-580. |
| Zheng S, Sun M, Zhang K*, Gu J, Guo Z, Tian S, Zhai G, He X,, Zhang Y*. |
Profiling post-translational modifications of histones in neural differentiation of embryonic stem cells using liquid chromatography-mass spectrometry. |
J Chromatogr B |
2016 |
1017-1018 :36-44. |
| Ju X, Hou Q, Sheng A, Wu K, Zhou Y,, Wen T, Yang Z, Wang X, Luo Z*. |
The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice. |
Elife |
2016 |
5: e18197-18222. |
| Liu J, Luo X, Xu Y, Gu J, Tang F, |
Single-stranded DNA binding protein Ssbp3 induces differentiation of mouse embryonic stem cells into trophoblast-like cells. |
Stem Cell Res Ther |
2016 |
7(1): 79-93. |
| Qu Z,GuanY,Cui L, Song J, Gu J, Zhao H, Xu L, Lu L, |
Transplantation of rat embryonic stem cell-derived retinal progenitor cells preserves the retinal structure and function in rat retinal degeneration. |
Stem Cell Research & Therapy |
2015 |
2015 6:219 |
| Sun M, Liao B, Tao Y, Chen H, Xiao F, Gu JJ, Gao SR and Jin Y* |
Calcineurin-NFAT Signaling Controls Somatic Cell Reprogramming in a Stage-Dependent Manner. |
Journal of Cellular Physiology |
2015 |
9999: 1–12 |
| Yu H, He K, Wang L, Hu J, Gu J, Zhou C, Lu R, |
Stk40 represses adipogenesis through translational control of CCAAT/enhancer-binding proteins. |
J Cell Sci. |
2015 |
128(15):2881-90. |
| Wei Z, Sun M, Liu X, Zhang J, |
Rufy3, a protein specifically expressed in neurons, interacts with actin-bundling protein Fascin to control the growth of axons. |
J Neurosci Res |
2014 |
92(5):607-18 |
| Zhu L, Zhang S, |
Foxd3 suppresses NFAT-mediated differentiation to maintain self-renewal of embryonic stem cells. |
EMBO Rep. |
2014 |
15(12):1286-96 |
| Luo X, Wang B, Tang F, Zhang J, Zhao Y, Li H, |
Wwp2 targets SRG3, a scaffold protein of the SWI/SNF-like BAF complex, for ubiquitination and degradation. |
Biochem Biophys Res Commun. |
2014 |
17;443(3):1048-53 |
| Liao B, Zhong X, Xu H, Xiao F, Fang Z, Gu J, Chen Y, Zhao Y, |
Itch, an E3 ligase of Oct4, is required for embryonic stem cell self-renewal and pluripotency induction. |
J Cell Physiol |
2013 |
228(7):1443-51 |
| Zhou Y, Jiang H, Gu J,Tang Y, Shen N and Jin Y*. |
MicroRNA-195 targets ADP-ribosylation factor like protein 2 inducing apoptosis in human embryonic stem cell-derived neural progenitor cells. |
Cell Death & Disease |
2013 |
4:e695 |
| Cui L, Guan Y, Qu Z, Zhang J, Liao B, Ma B, Qian J, Li D, Li W, Xu GT, |
WNT signaling determines tumorigenicity and function of ESC-derived retinal progenitors. |
J Clin Invest. |
2013 |
123(4):1647-61 |
| Yu H, He K, Li L, Sun L, Tang F, Li R, Ning W, |
Deletion of STK40 protein in mice causes respiratory failure and death at birth. |
J Biol Chem |
2013 |
288(8):5342-52 |
| Yang H, Liu Z, Ma Y, Zhong C, Yin Q, Zhou C, Shi D, Cai Y, Zhao H, Wang H, Tang F, Wang Y, Zhang C, Lai D,, Sun Q*, Li J* |
Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes |
Cell Res |
2013 |
23:1187-1200 |
| Guan Y, Cui L, Qu Z, Lu L, Wang F, Wu Y, Zhang J, Gao F, Tian H, Xu L, Xu G, Li W*, |
Subretinal transplantation of rat MSCs and erythropoietin gene modified rat MSCs for protecting and rescuing degenerative retina in rats |
Cur Mol Med |
2013 |
13:1419-1431 |
| Ma Y, Gu J, Li C, Wei X, Tang F, Shi G, Jiang J, Kuang J, Li J, Wang Z, Xie X , |
Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells |
Stem Cell Research & Therapy |
2012 |
3(4):29 |
| Ma Y, Li C, Gu J, Tang F, Li C, Li P, Ping P, Yang S, Li Z, |
Aberrant gene expression profilesin pluripotent stem cells induced from fibroblasts of a klinefelter syndrome patient |
J Biol Chem. |
2012 |
287(46): 38970–38979 |
| Zhang H, Ma Y, Gu J, Liao B, Li J, Wong J, |
Reprogramming of somatic cells via TAT-mediated protein transduction of recombinant factors |
Biomaterials |
2012 |
33(20): 5047- 5055 |
| Zhou Y, |
Differentiation of Human Embryonic Stem Cells into Neural Lineage Cells. In M.A. Hayat (ed.) |
Stem Cells and Cancer Stem Cells. |
2012 |
7:229-239 |
| Shi G,Gao F, |
The regulatory role of histone deacetylase inhibitors in Fgf4 expression is dependent on the differentiation state of pluripotent stem cells |
J Cell Physiol. |
2011 |
226:3190-3196. |
| Lu R, Yang A, |
Dual functions of T-box3 in the control of self-renewal and extraembryonic endoderm differentiation in mouse embryonic stem cells. |
J Biol Chem. |
2011 |
286 (10):8425-8436. |
| Yang A, Shi G, Zhou C, Lu R, Li H ,Sun L, |
Nucleolin Maintains Embryonic Stem Cell Self-renewal by Suppression of p53 Protein-dependent Pathway |
J Biol Chem. |
2011 |
286(50):43370-43382 |
| Li X, Zhu L, Yang A, Lin J, Tang F, Jin S, Wei Z, Li J, |
Calcineurin-NFAT signaling critical regulates early lineage specification in mouse embryonic stem cells and embryos |
Cell Stem Cell. |
2011 |
8:46-58. |
| Fang H, Yang Y, Li C, Fu S, Yang Z, Jin G, Wang K, Zhang J, |
Tranome analysis of early organogenesis in human embryos. |
Developmental Cell |
2010 |
19:174-184. |
| Li L, Sun L, Gao F, Jiang J, Yang Y, Li C, Gu J, Wei Z, Yang A, Lu R, Ma Y, Tang F, Kwon S, Zhao Y, Li S, |
Stk40 links the pluripotency factor Oct4 to the Erk/MAPK pathway and controls extraembryonic endoderm differentiation. |
PNAS |
2010 |
107 (4):1402-1407 |
| Shi G, |
Role of Oct4 in maintaining and regaining stem cell pluripotency. |
Stem Cell Research & Therapy |
2010 |
1(5): 39 |
| Li C, Yang Y, Lu X, Sun Y, Gu J, Feng Y, |
Efficient derivation of Chinese human embryonic stem cell lines from frozen embryos. |
In vitro Cell & Dev. Biol. –Animal |
2010 |
46:186-191. |
| Liao B, |
Wwp2 mediates Oct4 ubiquitination and its auto-ubiquitination in a dosage-dependent manner. |
Cell Res. |
2010 |
20(3):332-344. |
| Li C, Yang Y, Gu J, Ma Y, |
Derivation and tranional profiling analysis of pluripotent stem cell lines from rat blastocysts |
Cell Res |
2009 |
19:173-186 |
| Li C, Yu H, Ma Y, Shi G, Jiang J, Gu J, Yang Y, Jin S, Wei Z, Jiang H, Li J, |
Germline-competent mouse-induced pluripotent stem cell lines generated on human fibroblasts without exogenous leukemia inhibitory factor. |
PLoS One |
2009 |
4:e6724 |
| Chunliang Li ,, |
Pluripotency can be repidly and efficiently induced in human amniotic fluid-dsrived cells |
Human Molecular Genetics |
2009 |
15(22): 4340-9 |
| Li H, Wang B, Yang A, Lu R, Wang W, |
LYAR is an important nucleolar protein for control of self-renewal and differentiation in embryonic stem cells |
Stem Cells |
2009 |
5;27(6):1244-1254 |
| Xu H, Wang W, Li C, Yu H, Yang A, Wang B, |
WWP2 promotes degradation of tranion factor OCT4 in human embryonic stem cells |
Cell Res |
2009 |
19:561-573 |
| Zhong X, |
Critical roles of coactivator p300 in mouse embryonic stem cell differentiation and Nanog expression. |
Journal of Biological Chemistry |
2009 |
284 (14 ) :9186-9175 |
| Lingjie Li,Lei Sun, |
Stk40 links the pluripotency factor Oct4 to the Erk/MAPK Pathway and controls extraembryonic endoderm differentiation |
PNAS |
2009 |
107 (4):1402-1407 |
| Furong Gao, |
PARP1poly(ADP-ribosyl)ates Sox2 to control Sox2 protein levels and FGF4 expression during embryonic stem cell differentiation |
Journal of Biological Chemistry |
2009 |
14;284(33): 22263-73. |
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專利與技術許可 >>>
1. Oct4泛素化修飾突變體在提高誘導體細胞重編程效率中的應用,，申請?zhí)?/span>201810783982.4，金穎,，廖兵,，李爽，肖峰,； 2. 一種PRRT2蛋白相關的抗原表位肽及其應用,，申請?zhí)?/span>2014103873851，金穎,、李春,； 3. 一種調(diào)控誘導產(chǎn)生的視網(wǎng)膜前體細胞的方法,，ZL 2012 1 0279963.0，金穎,、徐國彤,、崔璐、管圓,； 4. 抗小鼠Stk40多克隆抗體及其制備,，ZL 2011 1 0214235.7，金穎,、楊阿聰,； 5. 調(diào)控胚胎干細胞及早期胚胎的胚層分化的方法和試劑，ZL 2010 1 0268307.1,，金穎,、李翔、朱莉莉,。 |
榮譽與獎勵 >>>
1. 2001年上海曙光學者,； 2. 2004年度獲得中國科學院“百人計劃”支持； 3. 2006年當選為上海市優(yōu)秀學科帶頭人,； 4. 2009年度第二屆百名華僑華人專業(yè)人士-杰出創(chuàng)業(yè)獎,； 5. 2009-2010年度獲上海市“三八”紅旗手稱號； 6. 2010年度中國科學院“朱李月華優(yōu)秀教師”獎,； 7. 2013年度,、2016年度中國科學院上海生命科學研究院/上海交通大學醫(yī)學院健康科學研究所“杰出研究員”獎； 8. 2011年度,、2012年度,、2017年度中國科學院“優(yōu)秀研究生指導教師”獎； 9. 2011年度,、2016年度中國科學院上海生命科學研究院/上海交通大學醫(yī)學院健康科學研究所優(yōu)秀共產(chǎn)黨員,； 10. “胚胎干細胞及早期胚胎發(fā)育的分子調(diào)控機制研究”獲得2012年教育部自然科學獎二等獎（第一完成人）； 11. “胚胎干細胞中Oct4轉(zhuǎn)錄因子的分子調(diào)控機制研究”獲得2013年獲得上海市科學技術獎三等獎（第一完成人）,； 12. “以多能干細胞為模型對細胞命運決定分子機制的研究”獲得2014年上海市自然科學獎二等獎（第一完成人）,； 13. 2017年、2018年實驗室獲得中國細胞生物學學會全國聯(lián)動實驗室開放日科普活動優(yōu)秀獎,； 14. 2017年中國細胞生物學學會干細胞生物學分會干細胞研究創(chuàng)新獎,； 15. 2018年度上海交通大學基礎醫(yī)學院優(yōu)秀員工獎。
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