李慶
組織胚胎學(xué)與遺傳發(fā)育學(xué)系
基本信息
李慶
原始生殖細(xì)胞與早期胚胎發(fā)育調(diào)控機(jī)制研究組
電話：
郵箱：[email protected]
研究方向
課題組聚焦原始生殖細(xì)胞（Primordial germ cell, PGC）與早期胚胎發(fā)育的交叉研究，這一階段是生命起源的關(guān)鍵窗口，涉及劇烈的表觀遺傳重編程和轉(zhuǎn)錄動(dòng)態(tài)調(diào)控。課題組將以基因編輯、半克隆技術(shù)、單細(xì)胞多組學(xué)、胚胎干細(xì)胞、小鼠模型、和臨床樣本等方法和材料，探究如下重要的科學(xué)問(wèn)題：
1）表觀遺傳調(diào)控因子家族在早期胚胎發(fā)育中的功能機(jī)制研究：系統(tǒng)解析表觀調(diào)控因子家族的主效及協(xié)同作用，構(gòu)建早期胚胎發(fā)育的表觀調(diào)控網(wǎng)絡(luò)圖譜；
2）PGC發(fā)育關(guān)鍵基因及功能位點(diǎn)的挖掘與機(jī)制研究：識(shí)別PGC發(fā)育過(guò)程中尚未定義的關(guān)鍵基因與功能核苷酸位點(diǎn)，特別聚焦于胚胎致死基因，揭示其調(diào)控機(jī)制及相互作用網(wǎng)絡(luò)；
3）PGC缺陷模型中異種嵌合技術(shù)構(gòu)建生殖細(xì)胞的研究：基于PGC發(fā)育缺陷的胚胎模型，探索通過(guò)異種嵌合實(shí)現(xiàn)高等生物功能性生殖細(xì)胞產(chǎn)生的新策略，為不孕不育治療提供新的突破路徑。
個(gè)人簡(jiǎn)歷
李慶博士2020年5月畢業(yè)于中國(guó)科學(xué)院分子細(xì)胞科學(xué)卓越創(chuàng)新中心獲得理學(xué)博士學(xué)位；2020年5月至2023年10月，在該中心開(kāi)展博士后研究，師從李勁松院士；2023年11月至2025年4月，任該中心副研究員。長(zhǎng)期致力于運(yùn)用基因編輯技術(shù)及類(lèi)精子干細(xì)胞介導(dǎo)的半克隆體系，系統(tǒng)解析關(guān)鍵功能基因及其位點(diǎn)在生殖細(xì)胞發(fā)育、胚胎形成以及相關(guān)疾病發(fā)生過(guò)程中的作用機(jī)制，為不孕不育和妊娠失敗的治療提供潛在靶點(diǎn)和理論依據(jù)。以第一作者或通訊作者（含共同）在Science（2021）、Nature Cell Biology（2018）、Nucleic Acids Research（2021）、Nature Communications（2021, 2023, 2024）、Cell Discovery（2023）和Science China Life Sciences（2020, 2025）等國(guó)際高水平期刊發(fā)表研究論文共11篇，并以合作作者在Nature Cell Biology等期刊發(fā)表論文9篇。主持國(guó)家自然科學(xué)基金面上項(xiàng)目、青年基金項(xiàng)目（C類(lèi)）、博士后創(chuàng)新人才計(jì)劃等多項(xiàng)國(guó)家級(jí)科研項(xiàng)目。2025年5月起，加入上海交通大學(xué)基礎(chǔ)醫(yī)學(xué)院，任組織胚胎學(xué)與遺傳發(fā)育學(xué)系/上海市生殖醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室課題組長(zhǎng)/研究員。
論文與專(zhuān)著
1) Yin, X#., Yan, M#., Cheng, Y., Li, Z., Cui, C., Wang, Y., Liu, N., Shu, Y., Li, J*. and Li, Q* (2025) Efficient generation of all ESC-derived mice carrying a homozygous lethal mutation through eight-cell embryo injection. Sci China Life Sci, 2025, 33(1): 1-4.
2) Li, Q#., J. Lu#, X. Yin#, Y. Chang#, C. Wang, M#. Yan, L#. Feng, Y. Cheng, Y. Gao, B. Xu, Y. Zhang, Y. Wang, G. Cui, L. Xu, Y. Sun, R. Zeng, Y. Li, N. Jing, G.-L. Xu, L. Wu, F*. Tang*, and J. Li*. 2023. Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation. Nature Communications. 14:2922.
3) Li, Q#,*., C. Cui#, R. Liao, X. Yin, D. Wang, Y. Cheng, B. Huang, L. Wang, M. Yan, J. Zhou, J. Zhao, W. Tang, Y. Wang, X. Wang, J. Lv, J. Li, H. Li*, and Y. Shu*. 2023. The pathogenesis of common Gjb2 mutations associated with human hereditary deafness in mice. Cellular and Molecular Life Sciences. 80:148.
4) Li, Q#., Li, Y#., Yang, S., Huang, S., Yan, M., Ding, Y., Tang, W., Lou, X., Yin, Q., Sun, Z., Lu, L., Shi, H., Wang, H., Chen, Y*. and Li, J*. (2018) CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nature Cell Biology 20(11), 1315-1325. Highlighted by NCB.
5) Li, Q#., Li, Y#., Yin, Q#., Huang, S#., Wang, K#., Zhuo, L., Li, W., Chang, B. and Li, J*. (2020) Temporal regulation of prenatal embryonic development by paternal imprinted loci. Sci China Life Sci 63(1), 1-17. Published as a Cover story, highlighted by Sci China Life Sci.
6) Kang, J. Y#., Wen, Z#., Pan, D#., Zhang, Y#., Li, Q#., Zhong, A#., Yu, X#., Wu, Y. C., Chen, Y., Zhang, X., Kou, P. C., Geng, J., Wang, Y. Y., Hua, M. M., Zong, R., Li, B., Shi, H. J., Li, D., Fu, X. D., Li, J., Nelson, D. L., Guo, X., Zhou, Y., Gou, L. T., Huang, Y*., and Liu, M. F*. (2022) LLPS of FXR1 drives spermiogenesis by activating translation of stored mRNAs. Science 377. Highlighted by Science.
7) Zhao, T#., Li, Q#., Zhou, C#., Lv, X., Liu, H., Tu, T., Tang, N., Cheng, Y., Liu, X., Liu, C., Zhao, J., Song, Z., Wang, H., Li, J*. and Gu, F*. (2021) Small-molecule compounds boost genome-editing efficiency of cytosine base editor. Nucleic Acids Res 49(15), 8974-8986.
8) Fu, J#., Li, Q#., Liu, X#., Tu, T#., Lv, X., Yin, X., Lv, J., Song, Z., Qu, J., Zhang, J., Li, J*. and Gu, F*. (2021) Human cell based directed evolution of adenine base editors with improved efficiency. Nature Communications 12(1), 5897.
9) Gu, W#., Zhang, J#., Li, Q#., Zhang, Y#,*., Lin, X#., Wu, B#., Yin, Q#., Sun, J., Lu, Y., Sun, X., Jia, C., Li, C., Zhang, Y., Wang, M., Yin, X., Wang, S., Xu, J., Wang, R., Zhu, S., Cheng, S., Chen, S., Liu, L., Zhu, L., Yan, C., Yi, C., Li, X., Lian, Q., Lin, G., Ling, Z., Ma, L., Zhou, M., Xiao, K., Wei, H., Hu, R*., Zhou, W*., Ye, L*., Wang, H*., Li, J*., and Sun, B*. (2023) The TRIM37 variants in Mulibrey nanism patients paralyze follicular helper T cell differentiation. Cell Discovery 9, 82.
10) Liu, Y#., Li, Q#., Yan, T., Chen, H., Wang, J., Wang, Y., Yang, Y., Xiang, L., Chi, Z., Ren, K., Lin, B., Lin, G., Li, J., Liu, Y*., and Gu, F*. (2023) Adenine base editor-mediated splicing remodeling activates noncanonical splice sites. The Journal of Biological Chemistry 299, 105442.
11) Huang, C#*., Zhu, W#., Li, Q#., Lei, Y#., Chen, X., Liu, S., Chen, D., Zhong, L., Gao, F., Fu, S., He, D., Li, J., and Xu, H*. (2024) Antibody Fc-receptor FcεR1γ stabilizes cell surface receptors in group 3 innate lymphoid cells and promotes anti-infection immunity. Nature Communications 15, 5981
12) Huang, C#,*., Li, Q#,*., and Li, J. (2022) Site-specific genome editing in treatment of inherited diseases: possibility, progress, and perspectives. Medical review 2, 471-500
科研項(xiàng)目
1）上海交通大學(xué)醫(yī)學(xué)院引進(jìn)人才啟動(dòng)基金，2025.5~2031.7，課題負(fù)責(zé)人
2）國(guó)家自然科學(xué)基金委，面上項(xiàng)目，2024.1~2027.12，課題負(fù)責(zé)人
3）國(guó)家科技重大專(zhuān)項(xiàng)科技創(chuàng)新2030-“癌癥、心腦血管、呼吸和代謝性疾病防治研究”重點(diǎn)專(zhuān)項(xiàng)，2025.1~2028.12，課題骨干
團(tuán)隊(duì)介紹
課題組將秉持嚴(yán)謹(jǐn)、創(chuàng)新、平等的科研理念，致力于揭示生命起源的分子基礎(chǔ)，為生殖發(fā)育起源疾病的防治提供前沿理論與技術(shù)支持。現(xiàn)因課題組發(fā)展需要，擬招聘助理研究員1名、實(shí)驗(yàn)技術(shù)員1名、博士后2-3名和意向報(bào)考本實(shí)驗(yàn)室的碩士/博士研究生，也非常歡迎聯(lián)合培養(yǎng)的研究生。有意向請(qǐng)聯(lián)系：[email protected]，期待您的加盟！
