生物傳感與環(huán)境健康課題組
Biosensing and Environmental Health
一、 PI介紹
Principal investigator
殷堃,，研究員,，上海市海外高層次人才。2016年博士畢業(yè)于中國科學(xué)院大學(xué),。2016-2021年分別在俄亥俄州立大學(xué),、賓夕法尼亞大學(xué)與康尼狄格大學(xué)擔(dān)任博士后研究員。主持了一系列新型,、快速,、超靈敏、高通量分析診斷技術(shù)及傳染性疾病移動(dòng)互聯(lián)智能檢測(cè)平臺(tái)的開發(fā)研究,。目前已發(fā)表SCI論文,、IEEE會(huì)議論文30余篇，其中在Chemical Engineering Journal（IF = 13.273）,、Theranostics（IF = 11.556）,、Water Research（IF = 11.236）、Biosensors and Bioelectronics（IF = 10.618）,、Analytical Chemistry（IF = 6.986）,、Lab on a Chip（IF = 6.799）中科院一區(qū)期刊上以第一作者身份發(fā)表SCI論文11篇，影響因子10以上論文6篇,，被他引1000余次,。此外以通訊作者出版學(xué)術(shù)專著2部，獲授權(quán)中國發(fā)明專利8項(xiàng),。曾獲得“中國科學(xué)院院長(zhǎng)特別獎(jiǎng)”,、“中國科學(xué)院優(yōu)秀博士生論文”等榮譽(yù)。
Professor Kun Yin obtained a Ph.D. degree from the University of Chinese Academy of Sciences in 2016 and completed a postdoctoral fellowship at Ohio State University, University of Pennsylvania and University of Connecticut, respectively between 2016-2021. His research interests focus on the development of novel, fast, ultra-sensitive, high-throughput analysis and diagnosis technologies and mobile Internet intelligent detection platform for infectious disease diagnosis. He has published more than 30 SCI papers and IEEE conference papers in total. Prof. Yin has published 11 SCI papers including Chemical Engineering Journal (IF = 13.273), Theranostics (IF = 11.556), Water Research (IF = 11.236), Biosensors and Sensors, Analytical Chemistry (IF = 6.986), and Lab on a Chip (IF = 6.799) as the first author. In addition, Prof. Yin has published two books as the corresponding author and has been authorized 8 Chinese invention patents. He has selected as "Special Award of President of Chinese Academy of Sciences" and "Excellent Doctoral Thesis of Chinese Academy of Sciences".
二,、 研究組介紹
Introduction
生物傳感與環(huán)境健康課題組主要在人類與環(huán)境界面上進(jìn)行生物安全風(fēng)險(xiǎn)防控與治理體系建設(shè),，提高國家生物安全治理能力，切實(shí)保護(hù)國民生命健康安全,。主要的研究方向?yàn)椴≡⑸餀z測(cè),、抗生素耐藥防控等。目前,，環(huán)境健康與生態(tài)安全系承擔(dān)國家級(jí),、部委級(jí),、校級(jí)等多項(xiàng)研究項(xiàng)目，在研經(jīng)費(fèi)近300萬,。研究組成員包括：胡沁沁,、修樂山。
Biosensing and Environmental Health research group focuses on biosafety risk prevention, control and management system construction at the human-environment interface, improves national biosafety management capacity, and effectively protects health safety of citizens. The research mainly focus on infectious disease diagnosis, antibiotic resistance prevention, etc. At present, the Department of Environmental Health and Ecological Security is in charge of a number of national, ministerial, university-level research projects, with nearly 3 million research funds. Members include Qin-qin Hu and Le-shan Xiu.
三,、 代表性科研成果介紹
Major Research Achievements of the Department
（一） 項(xiàng)目資助情況
Project funding
1. 國家自然科學(xué)基金青年項(xiàng)目：基于CRISPR/Cas識(shí)別機(jī)理的新型冠狀病毒多靶位可視化診斷方法研究（22104090）
Novel Coronavirus Multi-target visual diagnosis Method based on CRISPR/Cas recognition mechanism, 22104090, Youth Foundation of National Natural Science Foundation of China.
2. 轉(zhuǎn)化醫(yī)學(xué)國家重大科技基礎(chǔ)設(shè)施（上海）開放課題項(xiàng)目：級(jí)聯(lián)放大-適配體傳感器用于診斷耐藥型TNBC外泌體（TMSK-2021-114）
Aptasensors with cascades amplification for diagnosis of TNBC exosomes, TMSK-2021-114, National Facility of Translational Medicine (Shanghai).
3. 崇明島全健康先導(dǎo)項(xiàng)目研究：崇明島人-動(dòng)物-環(huán)境界面的廢水耐藥性監(jiān)測(cè)與控制（SGHKJCX20221-02）
Monitoring and control of wastewater resistance at human-animal-environment interface in Chongming Island, SGHKJCX20221-02, One Health Pilot Study on Chongming Island's.
4. 國家衛(wèi)生健康委寄生蟲病原與媒介生物學(xué)重點(diǎn)實(shí)驗(yàn)室開放課題基金：基于環(huán)境核酸的血吸蟲現(xiàn)場(chǎng)監(jiān)測(cè)預(yù)警技術(shù)研究：（NHCKFKT2021-06）
Research on on-site monitoring and early warning technology of Schistosoma japonicum based on environmental nucleic acid, NHCKFKT2021-06, Open Project Fund of Key Laboratory of Parasite And Vector Biology, NHC.
（二） 代表性論文
Selected Publications
1. Chen Chen, Qinqin Hu, Kun Yin*. Visible transparency tuning and corresponding sensing application of opal photonic crystals. Optics Express, 2021. (JCR 1區(qū))
2. Kun Yin, Xiong Ding, Zhiheng Xu, Ziyue Li, Xingyu Wang, Hui Zhao, Clifford Otis, Baikun Li, Changchun Liu*. Multiplexed colorimetric detection of SARS-CoV-2 and other pathogens in wastewater on a 3D printed integrated microfluidic chip. Sensors and Actuators B: Chemical, 2021, 130242. (IF=7.460)
3. Kun Yin, Xiong Ding, Ziyue Li, Maroun M. Sfeir, Enrique Ballesteros, Changchun Liu*. Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2. Lab on a Chip, 2021, (IF=6.799)
4. Kun Yin, Xiong Ding, Ziyue Li, Hui Zhao, Kumarasen Cooper, Changchun Liu*. Dynamic aqueous multiphase reaction system for one-pot CRISPR-Cas12a based ultrasensitive and quantitative molecular diagnosis. Analytical Chemistry, 2020, 92:8561-8568. (IF= 6.986)
5. Kun Yin, Vikram Pandian, Karteek Kadimisetty, Xin Zhang, Carlos Ruiz, Kumarasen Cooper, Changchun Liu*. Real-time colorimetric Quantitative Molecular Detection of infectious Diseases on Smartphone-based Diagnostic platform. Scientific Reports, 2020, 10：1-9. (IF= 4.379)
6. Kun Yin, Vikram Pandian, Karteek Kadimisetty, Carlos Ruiz, Kumarasen Cooper, Jianxin You, and Changchun Liu*. Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening. Theranostics, 2019, 9: 2637-2645. (IF= 11.556)
7. Kun Yin, Qiaoning Wang, Min Lv, Lingxin Chen*. Microorganism remediation strategies towards heavy metals. Chemical Engineering Journal, 2019, 360: 1553-1563. (IF= 13.273)
8. Kun Yin, Min Lv, Qiaoning Wang; Yixuan Wu; Chunyang Liao*, Lingxin Chen. Simultaneous Bioremediation and Biodetection of Mercury Ion through Surface Display of Carboxylesterase E2 from Pseudomonas Aeruginosa PA1. Water Research, 2016. 103: 383-390. (IF= 11.236)
9. Kun Yin, Yixuan Wu, Shasha Wang and Lingxin Chen*. A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine. Sensors and Actuators B: Chemical, 2016, 232: 257-263. (IF=7.460)
10. Kun Yin, Fabiao Yu, Dongyan Liu, Zhihong Xie and Lingxin Chen*. Cyanine-based colorimetric and fluorescent probe for the selective detection of diethylstilbestrol in seawater, shrimp and fish samples. Sensors and Actuators B: Chemical, 2016, 223: 799-805. (IF=7.460)
11. Kun Yin, Fabiao Yu*, Weiwei Zhang, and Lingxin Chen*. A near-infrared ratiometric fluorescent probe for cysteine detection over glutathione indicating mitochondrial oxidative stress in vivo. Biosensors and Bioelectronics, 2015, 74: 156-164. (IF=10.618)
12. Kun Yin, Bowei Li, Xiaochun Wang, Weiwei Zhang* and Lingxin Chen*. Ultrasensitive colorimetric detection of Cu2+ ion based on catalyticoxidation of L-cysteine. Biosensors and Bioelectronics, 2015, 64: 81-87. (IF=10.618)
13. Kun Yin, Weiwei Zhang and Lingxin Chen*. Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone. Biosensors and Bioelectronics, 2014, 51: 90-96. (IF=10.618)
14. Kun Yin, Leming Sun, Zhen Fan, Huacan Lin, Yujian Huang, Scott Galster, Jeff, Kuret, Douglas Scharre, Mingjun Zhang*. Fluorescent peptide nanoparticles to monitor Aβ oligomers. IEEE-NANO, 2017 IEEE 17th International Conference on Nanotechnology, 2017, 25-28.
15. Zhiheng Xu, Kun Yin, Xiong Ding, Ziyue Li, Xuanhao Sun, Baikun Li, Rajesh V. Lalla, Robert Gross, and Changchun Liu.An integrated E-Tube cap for sample preparation, isothermal amplification and label-free electrochemical detection of DNA. Biosensors and Bioelectronics. 2021,186:113306. (IF=10.618)
16. Xiong Ding, Kun Yin, Ziyue Li, Maroun M. Sfeir, and Changchun Liu. Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay. Biosensors and Bioelectronics. 2021, 184: 113218. (IF=10.618)
17. Karteek Kadimisetty, Kun Yin, Aoife M. Roche, Yanjie Yi, Frederic D. Bushman, Ronald G. Collman, Robert Gross, Liang Feng, and Changchun Liu. "An integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care." Analyst 2021, 146: 3234-3241. (IF=3.978)
18. Xiong Ding, Kun Yin, Ziyue Li, Rajesh V. Lalla, Enrique Ballesteros, Maroun M. Sfeir, Changchun Liu*. Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay. Nature Communications, 2020, 11:1-10. (IF=11.800)
19. Weiwei Zhang, Kun Yin, Bowei Li and Lingxin Chen*. A glutathione S-transferase from Proteus mirabilis involved in heavy metal resistance and its potential application in removal of Hg2+. Journal of Hazardous Materials, 2013, 261: 646-652. (IF=9.038)
20. Weiwei Zhang, Kun Yin and Lingxin Chen*. Bacteria-mediated bisphenol A degradation. Applied Microbiology and Biotechnology, 2013, 97: 5681-5689. (IF=3.670)
21. Huacan Lin, Kun Yin, Zhen Fan, Jianjie ma, Mingjun Zhang*. Integrating tea nanoparticle with short peptides to generate tunable hydrogel scaffolds for diabetic ulcer. IEEE-NANO, 2017 IEEE 17th International Conference on Nanotechnology
22. Xiong Ding, Kun Yin, Ju Chen, Kepeng Wang, Changchun Liu*. A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity. Chemical Communications. 2019;55(84):12623-6. (IF=6.164)
（三） 發(fā)明專利
Patents
1. 陳令新, 殷堃, 于法標(biāo), 陳浩, 張衛(wèi)衛(wèi). 一種基于花菁的有機(jī)化合物及其應(yīng)用（專利號(hào): CN104119263B）
2. 陳令新, 殷堃, 于法標(biāo), 張衛(wèi)衛(wèi), 陳浩, 劉萍. 基于花菁的有機(jī)化合物及其應(yīng)用（專利號(hào): CN104098500A）
3. 陳令新, 殷堃, 張衛(wèi)衛(wèi). 一種簡(jiǎn)便快速檢測(cè)L-半胱氨酸的方法（專利號(hào): CN103969253A）
4. 陳令新, 殷堃, 張衛(wèi)衛(wèi). 一種簡(jiǎn)便快速檢測(cè)Cu2+的方法（專利號(hào): CN103969254B）
5. 陳令新, 殷堃, 張衛(wèi)衛(wèi). 一種生物元件用于檢測(cè)痕量二價(jià)銅離子的方法及其應(yīng)用（專利號(hào)：CN104819972A）
6. 陳令新, 殷堃, 于法標(biāo). 一種基于花菁的探針用于檢測(cè)痕量二價(jià)銅離子的方法及其應(yīng)用（專利號(hào)：CN104849271B）
（四） 獲獎(jiǎng)及各級(jí)人才情況
Awards and talents at all levels
1. 2021,，上海市海外高層次人才（殷堃）
Kun Yin, Shanghai overseas high-level talents, 2021.
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